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大豆检测试剂盒

货号

SG6-EK-96

Soy ELISA Kit - 96 wells

Members of the legume family, soybeans and their derived ingredients contain allergenic proteins. For example, soybean sprouts, soy sauce, hydrolyzed soy protein, tofu, miso, and soy lecithin retain allergic IgE-binding activity. BioFront's MonoTrace ELISA kit accurately and precisely detects the presence of soy in a variety of matrices and on surfaces.

作为豆科植物,大豆及其衍生成分含有过敏性蛋白质。例如,大豆芽、酱油、水解大豆蛋白、豆腐、味噌和大豆卵磷脂都保留了过敏性IgE结合活性。BioFront的MonoTrace ELISA试剂盒能够准确且精确地检测各种基质和表面中的大豆成分。

ASSAY PERFORMANCE(分析性能)

Assay Time: 30 minutes post-extraction

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Number of Tests per Kit: 96

Limit of Detection: 0.16 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Soy (Soya)

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

实验总时间:抽提后仅需30分钟!

孵育时间:抽提,样品孵育,二抗孵育,底物孵育每步仅需10分钟。

每一试剂盒测试数量:96

*检测限:0.16 ppm

*定量范围:1至40 ppm

特异性:大豆

*=参数的计算根据10次代表性的数据得出,然而,使用者应当查阅附随的分析证书中的具体批次数值。

THE MONOTRACE ADVANTAGE(优势)

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Peanut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target intact Peanut allergen even post food processing effects
  • Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility of 'false positive' results
  • Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies开发了第  条完全以单克隆抗体为基础的检测试剂盒产品(MonoTrace ELISAs),检测各种食物基质中的致敏蛋白污染。MonoTrace ELISAs综合使用一系列精准确定的经过严格筛选过程后仔细挑选的单克隆抗体来实现:

  • 分析耐食物加工的主要过敏原
  • 特异性识别目标成分,从而消除交叉反应,以及减少“假阳性”结果的可能性
  • 在各种食品(食品基质干扰可以忽略不计)的检测中表现优异

CROSS-REACTIVITY(交叉反应)

The assay exhibits strong reactivity to soy (Glycine max). At concentrations as high as 100,000 ppm Chick pea/garbanzo bean (0.006%), garfava flour (0.0025%), peanut (0.0017%) and lentils (0.0010%) were minimally cross-reactive. No cross-reactivity was observed in other matrices tested including a large panel of tree nuts, legumes, seeds, flours, meats and spices.

该检测对大豆(Glycine max)表现出强烈的反应性。在高达100,000 ppm的浓度下,鹰嘴豆/鹰嘴豆(0.006%)、鹰嘴豆面粉(0.0025%)、花生(0.0017%)和扁豆(0.0010%)的交叉反应性极小。在其他测试的基质中,包括大量坚果、豆类、种子、面粉、肉类和香料,均未观察到交叉反应。

RECOVERY(回收率)

Recovery of spiked soy samples at various concentrations was efficient (>79%) when the following food matrices were assayed: chocolate, cereals, breads, and cake.

在检测以下食品基质时,不同浓度下添加大豆样品的回收率是高效的(>79%):巧克力、谷物、面包和蛋糕。

大豆检测试剂盒
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