Name: 花生检测试剂盒
Rating: 4.8 (93 reviews)

首页

关于我们

产品中心

食品过敏原ELISA试剂盒食品过敏原LFD检测卡生命科学·病毒科研试剂

合作伙伴

新闻中心

会员中心

联系我们

图文
详情

花生检测试剂盒

货号：

PA3-EK-96

产品详情

Peanut allergies can cause severe and even fatal reactions. Although peanuts are members of the legume family, afflicted individuals are prone to react to tree nuts. BioFront's MonoTrace ELISA kit accurately detects the presence of peanut allergen even in thermally processed food.
花生会导致很严重甚至致命的过敏反应。虽然花生是豆科植物家族的成员，但受影响的人同时很容易对坚果产生过敏反应。佰弗生物的MonoTrace ELISA试剂盒能够精准地检测出花生过敏原，即使是在热加工的食品中。

ASSAY PERFORMANCE（分析性能）
Assay Time: Now just 30 minutes post-extraction!
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.
Number of Tests per Kit: 96
Limit of Detection: 0.24 ppm
Range of Quantification: 1 to 40 ppm
Specificity: Peanut
实验总时间：现在抽提后仅需30分钟！
孵育时间：抽提，样品孵育，二抗孵育，底物孵育每步仅需10分钟。
每一试剂盒测试数量：96
*检测限：0.24 ppm
*定量范围：1至40 ppm
特异性：花生
*=参数的计算根据10次代表性的数据得出，然而，使用者应当查阅附随的分析证书中的具体批次数值。

THE MONOTRACE ADVANTAGE（优势）
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Peanut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
Target intact Peanut allergen even post food processing effects
Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility of 'false positive' results
Perform well in a wide variety of food products with negligible food matrix interference
BioFront Technologies开发了第一条完全以单克隆抗体为基础的检测试剂盒产品（MonoTrace ELISAs），检测各种食物基质中的致敏蛋白污染。MonoTrace ELISAs综合使用一系列精准确定的经过严格筛选过程后仔细挑选的单克隆抗体来实现：
分析耐食物加工的主要过敏原
特异性识别目标成分，从而消除交叉反应，以及减少“假阳性”结果的可能性
在各种食品（食品基质干扰可以忽略不计）的检测中表现优异

CROSS-REACTIVITY（交叉反应）
The assay exhibits strong reactivity to peanut (Arachis hypogaea). Nutmeg (0.0026%), cayenne pepper (0.0011%), and pumpkin seed (0.0025%) were minimally reactive in the assay. Given the reported adulteration of some commercial spices with peanut in recent years, it is possible that the reactivity observed in certain matrices results from similar circumstances and does not represent cross reactivity within the assay. At concentrations as high as 100,000 ppm, no cross reactivity was observed when testing a variety of other matrices, including tree nuts, legumes, seeds and flours.
该检测方法对花生（学名：Arachis hypogaea）表现出强烈的反应性。肉豆蔻（反应性为0.0026%）、辣椒（反应性为0.0011%）和南瓜籽（反应性为0.0025%）在检测中表现出极低的反应性。鉴于近年来一些商业香料被报告掺杂花生成分，某些基质中观察到的反应性可能是由于类似的情况导致的，并不一定代表检测方法本身的交叉反应。在高达100,000 ppm的浓度下，测试其他多种基质（包括树坚果、豆类、种子和面粉）时，未观察到交叉反应。
RECOVERY（回收率）
The MonoTrace Peanut ELISA kit is suitable for the qualitative or quantitative detection of peanut within formula, chocolate, confectionary, ice cream and food components such as flours and non-fat dry milk. Confectionaries, chocolate and ice cream extractions require the addition of 5% (w/v) non-fat dry milk (NFDM) to the extraction buffer. This extraction additive is necessary for samples containing polyphenols (which include chocolate, fruits, teas, coffee, red wine and soybean as well as others not listed here) and complex polysaccharides (which includes carrageenan and gums such as xanthan, guar, cellulose among others). Recoveries were acceptable, according to AOAC guidelines (J. AOAC Int. 2012). In summary, peanut was efficiently extracted and detected from diverse food matrices.
Other matrices may also be suitable for the MonoTrace Peanut ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). An internal validation using a peanut-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.
MonoTrace花生ELISA试剂盒适用于配方食品、巧克力、糖果、冰淇淋以及面粉和脱脂奶粉等食品成分中花生的定性或定量检测。糖果、巧克力和冰淇淋提取物需要在提取缓冲液中添加5%（w/v）脱脂奶粉（NFDM）。这种提取添加剂对于含有酚类物质（包括巧克力、水果、茶、咖啡、红酒、大豆以及未在此列出的其他物质）和复杂多糖（包括卡拉胶以及黄原胶、瓜尔胶、纤维素等胶类）的样品是必需的。根据AOAC指南（《J. AOAC Int. 2012》），回收率是可接受的。总之，花生从多种食品基质中被高效提取和检测。
其他基质可能也适用于MonoTrace花生ELISA试剂盒。请与BioFront Technologies技术支持团队联系（support@biofronttech.com），以确定您感兴趣的基质是否经过验证（交叉反应性和回收率）。建议使用花生添加样进行内部验证，以确保在从试剂盒获得定性或定量结果之前，蛋白质能够被高效回收。