Name: 黄热病抗体 YFV NS1 Mab
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黄热病抗体 YFV NS1 Mab

货号ITEM # BF-077：

100UG 500UG 1MG

货号ITEM # BF-087：

100UG 500UG 1MG

产品详情

According to the World Health Organization (WHO), the American tropics have been reinfested with Aedes aegypti, the mosquito known to carry arboviruses such as Yellow Fever. Most urban dwellers are vulnerable because of low immunization coverage and Latin America is now at greater risk of epidemics than at any time in the past 50 years. The BioFront Technologies line of anti-Yellow Fever virus monoclonal antibodies have been demonstratrd to produce reliable and trusted results for researchers leading the fight against this global issue.

根据世界卫生组织（WHO）的数据，美洲热带地区再次被埃及伊蚊感染，这种蚊子已知携带黄热病等类动物传播病毒。大多数城市居民因免疫接种率低而易受影响，拉丁美洲目前面临的流行病风险比过去50年任何时候都高。 BioFront Technologies系列的抗黄热病病毒单克隆抗体已被证明能为领导全球性问题的研究人员带来可靠且值得信赖的结果。

BioFront Technologies提供抗黄热病病毒NS1单克隆抗体，已被证明对Western blot、IF和ELISA检测有效。
Catalog Number: BF-077-100ug
Immunogen: Cell-culture derived viral lysate from strain Yellow Fever strain 17D
Purification: Purified by anion exchange column chromatography
Isotype: IgG2a
Purity: >90% by SDS-PAGE
Specificity: Yellow Fever virus NS1 protein
Origin: Mouse
Buffer/Composition: 15 mM potassium phosphate, 0.85% NaCl, 0.05% NaN3; pH 7.2
Volume: 100 ul (100 ug)
Concentration: 1 mg/ml
Storage:
Short Term (less than 6 months): 4oC
Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)
Working Dilutions:
Western Blot- 1:1000
IF- 1:200-1:1000
ELISA- to be determined by user
Shipping: Shipped on ice packs
图片：
Figure 1: Immunoflourescent staining with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.
GFP-tagged Yellow Fever-17D virus was used to infect Huh-7.5 (P33) cells for 48 hours. Cells were fixed and stained with BioFront's anti-YFV NS1 clone BF-077 @ 1:200 for 2 hours at RT. Secondary antibody (goat anti-mouse Alexa 594) was incubated for 30 minutes at @ RT. Left panel shows infection as identified by the BF-077 anti-YFV NS1 monoclonal antibody (red) and the right panel shows the overlap with the viral GFP tag.

Figure 2. Western blot with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.
Primary antibody was diluted 1:1000 and incubated overnight at 4 C while rocking. Secondary antibody (goat-anti-mouse IgG-HRP from Santa Cruz Biotech) was diluted 1:2000 and incubated 1 hour at RT. (-) = uninfected Huh 7.5 cell lysate; ( ) = YFV-infectedcelllysate collected 48-hours post-infection with Yellow Fevery-17D virus. Arrow points to 45kD band representing YFV NS1 protein.
Catalog Number: BF-087-100ug
Immunogen: Cell-culture derived viral lysate from strain Yellow Fever strain 17D
Purification: Purified by anion exchange column chromatography
Isotype: IgG2a
Purity: >90% by SDS-PAGE
Specificity: Yellow Fever virus NS1 protein
Origin: Mouse
Buffer/Composition: 15 mM potassium phosphate, 0.85% NaCl, 0.05% NaN3; pH 7.2
Volume: 100 ul (100 ug)
Concentration: 1 mg/ml
Storage:
Short Term (less than 6 months): 4oC
Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)
Working Dilutions:
Western Blot- 1:1000
IF- 1:200-1:1000
ELISA- to be determined by user
Shipping: Shipped on ice packs
图片：
Figure 1: Immunoflourescent staining with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-087.
GFP-tagged Yellow Fever-17D virus was used to infect Huh-7.5 (P33) cells for 48 hours. Cells were fixed and stained with BioFront's anti-YFV NS1 clone BF-087 @ 1:200 for 2 hours at RT. Secondary antibody (goat anti-mouse Alexa 594) was incubated for 30 minutes at @ RT. Left panel shows infection as identified by the BF-087 anti-YFV NS1 monoclonal antibody (red) and the right panel shows the overlap with the viral GFP tag.

Figure 2. Western blot with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-087.
Primary antibody was diluted 1:1000 and incubated overnight at 4 C while rocking. Secondary antibody (goat-anti-mouse IgG-HRP from Santa Cruz Biotech) was diluted 1:2000 and incubated 1 hour at RT. (-) = uninfected Huh 7.5 cell lysate; ( ) = YFV-infectedcelllysate collected 48-hours post-infection with Yellow Fevery-17D virus. Arrow points to 45kD band representing YFV NS1 protein.
*The above images are property of BioFront Technologies Inc., all rights reserved.